Agilent AriaMx Real-Time PCR System
Agilent AriaMx Real-time PCR System
The Agilent AriaMx Real-time PCR System is an integrated qPCR platform designed for amplification, detention, and data analysis of genetic material. The system has features like a novel thermal cycler, an advanced optical system with LED excitation sources, and comprehensive data analysis software. Supporting a wide range of fluorescence detection techniques. Making it a versatile resource for various qPCR applications.
Features
- 96 wells
- 10-30 µL of reaction mixture per well
- Gene expression analysis
- New genotyping/HRM capability, mRNA quantification
- NGS quantification: library preparation, result validation, nucleic acid monitoring, rare allele detection
- Operates at a Cq uniformity standard deviation of <0.20 at fast cycling (5 sec at 95 °C / 10 sec at 60 °C)
- Data acquisition time of <3 sec/plate
- Dynamic range of 9 orders of magnitude
Currently available optical modules
FAM Module: (equivalent to SYBR Green)
- Detecting SYBR Green I and FAM dye
- Commonly used in quantitative PCR (qPCR) to monitor DNA amplification in real-time
- Frequently utilized in TaqMan probe based assays
- EM Wavelength 516.0 nm
- EX Wavelength 462.0 nm
HEX Module: (equivalent to JOE, VIC, BODIPY)
- Detects Hex dye, a green/ yellow fluorescent dye
- Used for manipulating, allowing detection of a second target alongside FAM
- Often applied in genotyping and mutation detection
- EM Wavelength 555.0 nm
- EX Wavelength 535.0 nm
ROX Module: (equivalent to Texas Red)
- Detects ROX dye, a passive reference dye
- Helps normalize fluorescence intensity across wells to correct for pipetting errors or volume variations
- Commonly used in qPCR to improve accuracy and precision
- EM Wavelength 610.0 nm
- EX Wavelength 585.0 nm
Optical modules available for purchase
CY5: (equivalent to APC, Alexa Fluor 647)
- EM Wavelength 665.0 nm
- EX Wavelength 635.0 nm
CY3: (equivalent to TAMRA, TET)
- EM Wavelength 568.5 nm
- EX Wavelength 542.0 nm
ATTO425: (equivalent to Alexa Fluor 430)
- EM Wavelength 475.0 nm
EX Wavelength 435.0 nm