Allan M. Showalter

Professor

Porter 504

Molecular Cellular Biology

Nanoscale Quantum Phenomena Institute (NQPI)

Plant Biology

showalte@ohio.edu

740-593-1135

Education

Ph.D., Rutgers University, 1983

Postdoctoral (Plant Molecular Biology), Washington University, St. Louis, MO 1983-1986

Ph.D. (Biochemistry), Rutgers University and the University of Medicine and Dentistry of New Jersey, Rutgers Medical School, New Brunswick, NJ 1983

B.A. (Biology), Franklin & Marshall College, Lancaster, PA 1978

Research

Molecular Biology and Biochemistry

Research Interests

Structure, biosynthesis and function of plant cell wall hydroxyproline-rich glycoproteins as revealed by molecular biology and biochemical studies. Particular interest in the extensins, solanaceous lectins, and arabinogalactan-proteins. Additional interests in plant DNA barcoding and the molecular and biochemical mechanisms of salt tolerance utilized by halophytes.

Structure, Expression and Function of Plant Cell Wall Proteins

Research in my lab is directed at elucidating the structure, expression, and function of the superfamily of hydroxyproline-rich glycoproteins (HRGPs) found in plant cell walls. HRGPs represent the major protein components of the plant cell surface (i.e., the plant cell wall and plasma membrane) and include the arabinogalactan-proteins (AGPs), extensins (EXTs), proline-rich proteins (PRPs), and solanaceous lectins. Using a variety of molecular biology techniques, we have isolated and characterized a number of cDNA and genomic clones encoding these cell surface proteins. Moreover, we have used these clones as molecular probes to examine the regulated expression of these genes during development and in response to various stress conditions such as wounding, pathogen infection, and drought. Biochemical studies are underway to determine the molecular interactions that these proteins undergo once deposited at the cell surface. Current efforts to alter the expression of these HRGPs in plants using a variety of techniques (antisense RNA, RNA interference, insertional mutagenesis, overexpression, expression of fusion proteins) in transgenic plants is also underway in order to elucidate their expression, molecular interactions, and functions. We have expressed a GFP-arabinogalactan-protein fusion in transgenic plants and used it to demonstrate that this particular arabinogalactan-protein is attached to the outer leaflet of the plasma membrane via a glycosylphosphatidylinositol (GPI) lipid anchor. We have also established a link between arabinogalactan-proteins and programmed cell death in plants. Our recent work makes use of the genetic model plant, Arabidopsis thaliana, in which we are studying various AGP mutants to elucidate AGP function and also isolating the enzymes (and the genes) responsible for glycosylating AGPs and other HRGPs. Our research on arabinogalactan-proteins uses many experimental approaches (i.e., molecular genetics, molecular biology, biochemistry, functional genomics, cell biology, plant physiology) and is supported by NSF and USDA grants.

Bioinformatics of Plant Cell Wall Proteins

Research in my lab also uses a bioinformatic approach to the identification and characterization of plant cell wall hydroxyproline-rich glycoproteins (HRGPs). Initially, Arabidopsis was used as the model organism for this work; now other plant genomes/proteomes are being examined. This bioinformatics work complements the lab bench work that we do on HRGPs and relates to the evolution of the HRGP superfamily and to the evolution of plants.

Molecular Adaptations of Halophytes to Saline Environments

Another project in the lab involves examining the molecular adaptations of halophytes to saline environments. Here, Atriplex prostrata, a plant that thrives in saline environments, is being examined with respect to its physiological and molecular responses to growth under various salt concentrations. We are particularly interested in the role which glycinebetaine plays as an osmoprotectant. Consequently, we have cloned the genes that encode the pathway for the synthesis of glycinebetaine for the purpose of genetically engineering crop plants to survive in saline soils.

Agricultural Nanofertilizers to Enhance Growth and Drought Tolerance of Plants

A new project in the lab involves testing the hypothesis that agricultural nanofertilizers can improve the growth and drought tolerance of plants in order to feed the ever increasing human population and deal with climate change. Specific nanofertilizers will be administered to plants grown in environmental chambers under normal conditions and under drought conditions. Plants grown with standard chemical fertilizers will be used as controls. Plants will be monitored weekly for standard growth and developmental parameters (i.e., germination time, germination efficiency, plant height, leaf number, root length, flowering time, fruiting time, total seed yield, etc.), and subjected to statistical analysis to determine differences among the plant groups. We hypothesize that the plants grown with one or more of the nanofertilizers will show superior growth and productivity, particularly under drought conditions. Future work will involve producing and testing additional nanofertilizers, conducting greenhouse and field studies, and expanding work to include additional crop plants.

Medicinal Plants: DNA Barcoding and Drug Discovery

My lab is also involved with another project using DNA Barcoding to uniquely identify medical plants that are used in Pakistan and elsewhere in the world. DNA is extracted from such plants provided by our Pakistani collaborators; then particular chloroplast DNA sequences (e.g. rbcL and matK) are amplified using the polymerase chain reaction (PCR) and sequenced to generate a DNA Barcode. This work is designed to verify that the proper medicinal plants are being used by medicinal plant industries and to insure that samples are not contaminated or adulterated with undesirable or harmful plant species. This work is now being expanded to include the extraction and identification of bioactive compounds from selected medicinal plants to treat human diseases, such as cancer and diabetes.

Faculty Research Focus Area: Plant Cell Wall Biotechnology

Current and Recent Research Projects

Isolation and characterization of the enzymes and genes responsible for glycosylating arabinogalactan-proteins (AGPs) and other hydroxyproline-rich glycoproteins (HRGPs).
A bioinformatics approach to the identification, classification, and analysis of plant cell wall HRGPs.
Molecular, cellular, biochemical, and physiological characterization of genetic mutants in AGP genes and AGP glycosyltransferase genes to elucidate AGP function.
DNA barcoding of medicinal plants used in Pakistan.

Courses Taught

PBIO 4500/5500 (Biotechnology and Genetic Engineering)
MCB 7200 (Molecular Biology)
MCB 7300 (Molecular and Cellular Biology Laboratory)
PBIO 3300/5300 (Plant Genetics)
PBIO 4270/5270 (Molecular Genetics)
BIOL 1010 (Principles of Biology)
PBIO 6910 (Seminar)
Plant Cell Wall Club

Professional Experience

2011-2012 Chair, Department of Environmental and Plant Biology, Ohio University, Athens, OH
1999-2000 Senior Fellow, School of Botany, The University of Melbourne, 1999-2000
1998-present Professor, Department of Environmental and Plant Biology and the Molecular and Cellular Biology Program, Ohio University, Athens, OH
1993-1996 Chair, Molecular and Cellular Biology Program, Ohio University, Athens, OH
1992-1998 Associate Professor, Department of Environmental and Plant Biology and the Molecular and Cellular Biology Program, Ohio University, Athens, OH
1986-1992 Assistant Professor, Department of Environmental and Plant Biology and the Molecular and Cellular Biology Program, Ohio University, Athens, OH
1983-1986 Research Associate (postdoctoral fellow) at Washington University, St. Louis, MO in the laboratory of Dr. Joseph E. Varner studying hydroxyproline-rich glycoproteins (extensins)
1979-1983 Research Assistantship at the Rutgers Medical School to isolate and characterize chick cDNAs coding for types I and II procollagen under the direction of Dr. Bjorn R. Olsen
1979 Teaching Assistantship for an introductory undergraduate Biochemistry course and a graduate Biochemistry course
1977-1978 Undergraduate senior research thesis on phytochrome control of hydroxyproline metabolism in Pisum sativum under the direction of Dr. Carl S. Pike

Awards and Honors

Invited to serve on the Scientific Session Committee for the XIII Cell Wall Meeting, Crete 2016
Invited Speaker at the XIV International Plant Cell Wall Meeting, Crete 2016
Invited Speaker at the 2nd International Conference on Salt Tolerance, Doha, 2013
Invited Speaker at the 6th International Barcode of Life Conference, Guelph, 2015
Invited Speaker at the at the Gordon Research Conference on Plant Cell Walls, Boston, 2015
Invited Speaker at the 5th International Barcode of Life Conference, Kunming, 2013
Invited Speaker at the XIII International Plant Cell Wall Meeting, Nantes 2013
Invited Speaker at the Society for Experimental Biology Meeting, Valencia 2013
Invited Speaker at the Pakistan-US Science and Technology Cooperation Program Technology Transfer Symposium, Islamabad, 2013
Invited Session Organizer and Speaker at the International Botanical Congress, Melbourne 2011
Co-Organizer and Speaker at an international workshop on “Medicinal Plants: Conservation and Sustainable Use”, Islamabad 2011
Invited Speaker at the Pakistan-U.S. Science and Technology Conference in Dubai, 2011
Invited to serve on the Scientific Organizing Committee for the XII Cell Wall Meeting, Porto 2010
Elected Chair (2009-2011) and Vice Chair (2008-2009), Ohio Plant Biotechnology Consortium
Invited Speaker at the XI International Plant Cell Wall Meeting, Copenhagen 2007
Invited Session Organizer and Speaker at the International Botanical Congress, Vienna 2005
Elected to serve on the American Society of Plant Biologists (ASPB) Executive Committee (2004-2007) and as the ASPB Midwest Section representative (2004-2007)
Invited Speaker at the X International Plant Cell Wall Meeting, Sorrento 2004
Invited Speaker at the Gordon Research Conference on Plant Cell Walls, 2003
Chair (2003-04) & Vice-chair (2002-03), American Society of Plant Biologists (Midwest Section)
Executive Committee for the Ohio Plant Biotechnology Consortium (OPBC), 2001-present
Invited Speaker at the IX International Plant Cell Wall Meeting, Toulouse 2001
Invited to serve on the Scientific Organizing Committee for the IX Cell Wall Meeting, Toulouse 2001
Served as editor and contributor to a collection of plant cell wall proteins manuscripts published by Cellular and Molecular Life Sciences, 2000-2001
Invited Instructor for an Advanced Course on Stress, Programmed Cell Death and Signaling in Plants & Speaker for a Cell Death Workshop, Instituto de Biologia Molecular e Celular, Porto 2000
Appointed Honorary Senior Fellow, School of Botany, The University of Melbourne, 1999-2000
Invited Session Organizer and Speaker at the International Botanical Congress, St. Louis 1999
Invited Speaker and Discussion Leader at the 20th Symposium in Plant Physiology, 1999
Invited Speaker at the VIII International Plant Cell Wall Meeting, Norwich 1998
Invited Session Chair and Speaker at the Gordon Research Conference on Plant Cell Walls, 1997
Invited Speaker at the Keystone Symposium on The Extracellular Matrix of Plants, 1996
Invited Speaker at the VII International Plant Cell Wall Meeting, Santiago de Compostela 1995
Invited Session Organizer and Speaker at the International Botanical Congress, Tokyo/Yokohama 1993
Invited Speaker at the VI International Plant Cell Wall Meeting, Nijmegen 1992
Invited Speaker at the Third International Congress of Plant Molecular Biology on "Molecular Biology of Higher Plant HRGPs", Tucson 1991
Invited Speaker at the Annual Meeting of the American Society of Plant Physiologists on "Extensin Family Members and Their Relatives", Albuquerque 1991
Invited Speaker at the American Society of Cell Biology Conference on "Biology of Plant and Animal Extracellular Matrix", 1990
Invited Speaker at the Gordon Research Conference on "Plant Molecular Biology", 1987
Invited Speaker at a Univ. of Wisconsin symposium on "Plant Genes of Economic Interest", 1986
Invited Speaker at the NATO Advanced Research Workshop on "Biology and Molecular Biology of Plant-Pathogen Interactions", Ilminster, U.K. 1985
Johnson & Johnson Fellowship (Competitive), 1981-1982
Graduated cum laude from Franklin & Marshall College with Honors in Biology, 1978

Research Publications

Showalter, A. M. and Basu, D. (2016) Extensin and arabinogalactan-protein biosynthesis: glycosyltransferases, research challenges, and biosensors. Frontiers in Plant Science 7, 814.
Showalter, A. M. and Basu, D. (2016) Glycosylation of arabinogalactan-proteins essential for development in Arabidopsis. Communicative & Integrative Biology 9, 3, e1177687.
Liu, X., R. Wolfe, L. R. Welch, D. S. Domozych, Z. A. Popper, and A. M. Showalter (2016) Bioinformatic identification and analysis of extensins in the plant kingdom. PLoS ONE, 11(2), e0150177.
Basu, D., L. Tian, T. Debrosse, E. Poirier, K. Emch, H. Herock, A. Travers, and A. M. Showalter (2016) Glycosylation of a fasciclin-like arabinogalactan-protein (SOS5) mediates root growth and seed mucilage adherence via a cell wall receptor-like kinase (FEI1/FEI2) pathway in Arabidopsis. PloS ONE, 11(1), e0145092.
Basu, D., L. Tian, W. Wang, S. Bobbs, H. Herock, A. Travers, and A. M. Showalter (2015) A small multigene hydroxyproline-O-galactosyltransferase family functions in arabinogalactan-protein glycosylation, growth and development in Arabidopsis. BMC Plant Biology 15(1), 1.
Basu, D., W. Wang, S. Ma, T. Debrosse, E. Poirier, K. Emch, E. Soukup, L. Tian, and A. M. Showalter (2015) Two hydroxyproline galactosyltransferases, GALT5 and GALT2, function in arabinogalactan-protein glycosylation, growth and development in Arabidopsis. PLoS ONE 10(5), e0125624.
Held, M.A., N. Jiang, D. Basu, A. M. Showalter, and A. Faik (2015) Plant cell wall polysaccharides: structure and biosynthesis [PDF]. In Polysaccharides, K.G. Ramawat, J.-M. Mérillon (eds.), Springer International Publishing Switzerland, pp. 3-54.
Liang Y., B. Basu, S. Pattathil, W.-l. Xu, A. Venetos, S. L. Martin, A. Faik, M. G. Hahn and A. M. Showalter (2013) Biochemical and physiological characterization of fut4 and fut6 mutants defective in arabinogalactan-protein fucosylation in Arabidopsis. Journal of Experimental Botany 64, 5537-5551.
Basu, D., Y. Liang, X. Liu, K. Himmeldirk, A. Faik, M. Kieliszewski, M. Held, and A.M. Showalter (2013) Functional identification of a hydroxyproline-O-galactosyltransferase specific forarabinogalactan protein biosynthesis in Arabidopsis. J. Biol. Chem. 288, 10132–10143.
Schori, M., M. Appel, A. Kitko, and A.M. Showalter (2013) Engineered DNA polymerase improves PCR results for plastid DNA. Applications in Plant Sciences 1, 1-7.
Lichtenberg, J., B.D. Keppler, T. Conley, D. Gu, P. Burns, L.R. Welch, and A.M. Showalter (2012) Prot-Class: a bioinformatics tool for protein classification based on amino acid signatures. Natural Science 4, 1161-1164.
Tan L., A.M. Showalter, J. Egelund, A. Hernandez-Sanchez, M.S. Doblin, and A. Bacic. (2012) Arabinogalactan-proteins and the research challenges for these enigmatic plant cell surface proteoglycans. Frontiers in Plant Science 3, 1-10.
Schori, M. and A.M. Showalter (2011) DNA barcoding as a means for identifying medicinal plants of Pakistan. Pakistan Journal of Botany 43, 1-4.
Zhang, Y., J. Yang, and A.M. Showalter (2011) AtAGP18, a lysine-rich arabinogalactan protein in Arabidopsis thaliana, functions in plant growth and development as a putative co-receptor for signal transduction. Plant Signaling and Behavior 6, 855-857.
Yang, J., Y. Zhang, Y. Liang, and A.M. Showalter (2011) Expression analyses of AtAGP17 and AtAGP19, two lysine-rich arabinogalactan-proteins, in Arabidopsis. Plant Biology, 13, 431-438.
Zhang, Y., J. Yang, and A.M. Showalter (2011) AtAGP18 is localized to the plasma membrane and functions in plant growth and development. Planta, 233, 675–683.
Yang, J. and A.M. Showalter (2010) Subcellular localization of a classical arabinogalactan-protein from Arabidopsis. Journal of Fuzhou University (Natural Science) 38, 753-757.
Liang, Y., A. Faik, M. Kieliszewski, L. Tan, W.-L. Xu, and A.M. Showalter (2010) Identification and characterization of in vitro galactosyltransferase activities involved in arabinogalactan-protein glycosylation in tobacco and Arabidopsis. Plant Physiol. 154, 632-642.
Keppler, B. and A.M. Showalter (2010) IRX14 and IRX14-LIKE, two glycosyl transferases involved in glucuronoxylan biosynthesis and drought tolerance in Arabidopsis. Molecular Plant 3, 834-841.
Showalter, A.M., B. Keppler, J. Lichtenberg, D. Gu, and L.R.Welch (2010) A bioinformatics approach to the identification, classification, and analysis of hydroxyproline-rich glycoproteins. Plant Physiol. 153, 485-513.
Wu Y., M. Williams., S. Bernard, A. Driouich, A.M. Showalter, and A. Faik. (2010) Functional identification of two non-redundant Arabidopsis α(1,2)fucosyltransferases specific to arabinogalactan-proteins. J. Biol. Chem. 285, 13638–13645.
Xu, J., L. Tan, D.T.A. Lamport, A.M. Showalter, and M.J. Kieliszewski (2008) The O-Hyp glycosylation code in tobacco and Arabidopsis and a proposed role of Hyp-glycans in secretion. Phytochemistry 69, 1631-1640.
Yang J. and A.M. Showalter (2007) Expression and localization of AtAGP18, a lysine-rich arabinogalactan-protein in Arabidopsis. Planta, 226, 169-179.
Sardar H. and A.M. Showalter (2007) A cellular networking model involving interactions among glycosylphosphatidylinositol (GPI)-anchored plasma membrane arabinogalactan proteins (AGPs), microtubules and F-actin in tobacco BY-2 cells. Plant Signaling and Behavior 2, 8-9.
Yang J., H.S. Sardar, K.R. McGovern, Y. Zhang, and A.M. Showalter (2007) A lysine-rich arabinogalactan-protein in Arabidopsis is essential for plant growth and development, including cell division and expansion. Plant Journal 49, 629-640.
Sardar, H., J. Yang, and A.M. Showalter (2006) Molecular interactions of arabinogalactan- proteins (AGPs) with cortical microtubules and actin in bright yellow 2 (BY-2) tobacco cultured cells. Plant Physiology 142, 1469-1479.
Lamport, D.T.A., M.J. Kieliszewski, and A.M. Showalter (2006) Salt stress upregulates periplasmic arabinogalactan proteins: using salt stress to analyse AGP function. New Phytologist 169, 479-492.
Wang, L.-W., A.M. Showalter, and I.A. Ungar (2005) Effects of intraspecific competition on growth and photosynthesis of Atriplex prostrata [PDF]. Aquatic Botany 83, 187-192.
Khan, M.A., I.A. Ungar, and A.M. Showalter (2005) Salt stimulation and tolerance in an intertidal stem-succulent halophyte [PDF]. Journal of Plant Nutrition 28, 1365-1374.
Sun, W., J. Xu, J. Yang, M.J. Kieliszewski, and A.M. Showalter (2005) The lysine-rich arabinogalactan-protein subfamily in Arabidopsis: gene expression, glycoprotein purification and biochemical characterization [PDF]. Plant and Cell Physiology 46, 975-984.
Sun, W., M.J. Kieliszewski, and A.M. Showalter (2004) Overexpression of tomato LeAGP-1 arabinogalactan-protein promotes lateral branching and hampers reproductive development [PDF]. Plant Journal 40, 870-881.
Wang, L.-W. and A.M. Showalter (2004) Cloning and salt-induced, ABA-independent expression of choline mono-oxygenase in Atriplex prostrata [PDF]. Physiologia Plantarum 120, 405-412.
Sun, W., Z.D. Zhao, M.C. Hare, M.J. Kieliszewski, and A.M. Showalter (2004) Tomato LeAGP-1 is a plasma membrane-bound glycosylphosphatidylinositol-anchored arabinogalactan-protein [PDF]. Physiologia Plantarum 120, 319-327.
Chaves I., A. Regalado, M. Chen, C. Ricardo, and A.M. Showalter (2002) Programmed cell death induced by (b-D-galactosyl)3 Yariv reagent in Nicotiana tabacum BY-2 suspension-cultured cells [PDF]. Physiologia Plantarum 116, 548-553.
Zhao Z., L. Tan, A.M. Showalter, D.T.A. Lamport, and M. Kieliszewski (2002) Tomato LeAGP-1 arabinogalactan-protein purified from transgenic tobacco corroborates the Hyp contiguity hypothesis [PDF]. Plant Journal 31, 431-444.
Showalter A.M. (2001) Introduction: plant cell wall proteins [PDF]. Cellular and Molecular Life Sciences 58, 1361-1362.
Showalter A.M. (2001) Arabinogalactan-proteins: structure, expression, and function [PDF]. Cellular and Molecular Life Sciences 58, 1399-1417.
Lu, H., M. Chen, and A.M. Showalter (2001) Developmental expression and perturbation of arabinogalactan-proteins during seed germination and seedling growth in tomato [PDF]. Physiologia Plantarum 112, 442-450.
Stratford, S., W. Barnes, D.L. Hohorst, J.G. Sagert, R. Cotter, A. Golubiewski, A.M. Showalter, S. McCormick, and P. Bedinger (2001) A leucine-rich repeat region is conserved in pollen extensin-like (Pex) genes in monocots and dicots [PDF]. Plant Mol. Biol. 46, 43-56.
Khan, M.A., I.A. Ungar, and A.M. Showalter (2000) Effects of sodium chloride treatments on growth and ion accumulation of the halophyte Haloxylon recurvum [PDF]. Communications in Soil Science and Plant Analysis 31, 2763-2774.
Showalter, A.M., M. Gao, M.J. Kieliszewski, and D.T.A. Lamport (2000) Characterization and localization of a novel tomato arabinogalactan-protein (LeAGP-1) and the involvement of arabinogalactan-proteins in programmed cell death [PDF]. In Cell and Developmental Biology of Arabinogalactan-Proteins, (E. A. Nothnagel, A. Bacic, and A. E. Clarke, eds.), Kluwer Academic/Plenum Publishers: New York, pp. 61-70.
Khan, M.A., I.A. Ungar, and A.M. Showalter (2000) The effect of salinity on the growth, water status, and ion content of a leaf succulent perennial halophyte, Suaeda fruticosa [PDF] (L.) Forssk. Journal of Arid Environments 45, 73-84.
Khan, M.A., I.A. Ungar, and A.M. Showalter (2000 Effects [PDF] of salinity on growth, water relations and ion accumulation of the subtropical perennial halophyte, Atriplex griffithii Moq. var. stocksii [PDF]. Annals of Botany 85, 225-232.
Gao, M. and A.M. Showalter (2000) Immunolocalization of LeAGP-1, a modular arabinogalactan-protein, reveals its developmentally regulated expression in tomato [PDF]. Planta 210, 865-874.
Gao, M. and A.M. Showalter (1999) Yariv reagent treatment induces programmed cell death in Arabidopsis cell cultures and implicates arabinogalactan-protein involvement [PDF]. Plant J. 19, 321-331.
Gao, M., M.J. Kieliszewski, D.T.A. Lamport, and A.M. Showalter (1999) Isolation, characterization, and immunolocalization of a novel, modular tomato arabinogalactan-protein corresponding to the LeAGP-1 gene [PDF]. Plant J. 18, 43-55.
Khan, M.A., I.A. Ungar, and A.M. Showalter (1999) Effects of salinity on growth, ion content, and osmotic relations in Halopyrum mucronatum [PDF] (L.) Stapf. J. Plant Nutr. 22, 191-204.
Khan, M.A., I.A. Ungar, A.M. Showalter, and H. Dewald (1998) NaCl-induced accumulation of glycinebetaine in four subtropical halophytes from Pakistan [PDF]. Physiol. Plant. 102, 487-492.
Wang, L.-W., A.M. Showater, and I.A. Ungar (1997) Effect of salinity on growth, ion content and cell wall chemistry in Atriplex prostrata Boucher [PDF]. Amer. J. Bot. 84, 1247-1255.
Li, S. and A.M. Showalter (1996) Cloning and developmental/stress-regulated expression of a gene encoding a tomato arabinogalactan protein [PDF]. Plant Molecular Biology 32, 641-652.
Showalter, A.M. (1993) Structure and function of plant cell wall proteins [PDF]. Plant Cell 5, 9-23.

Much of this research was supported by the National Science Foundation (NSF) under grants IBN-0110413 and IBN-9727757; however, “any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.”

Book Publications

Showalter, A. M. and K. M. Thompson (2016) BIOLOGY 1010 LABORTORY MANUAL – 3rd Edition. Van-Griner Publishing, Cincinnati, Ohio, ISBN: 978-1-61740-329-3, 157 pp.
Showalter, A. M. (2014) BIOLOGY 1010 LABORTORY MANUAL – 2nd Edition. Van-Griner Publishing, Cincinnati, Ohio, ISBN: 978-1-61740-131-2, 152 pp.
Showalter, A. M. (2013) BIOLOGY 1010 LABORTORY MANUAL. Van-Griner Publishing, Cincinnati, Ohio, ISBN: 978-1-61740-070-4, 154 pp.

Departmental Service

Promotion & Tenure Committee, Chair
Evaluation Committee, Chair

Scientific Societies & Board Memberships

Professional Scientific Society Memberships

American Association for the Advancement of Science (AAAS)
American Society of Plant Biologists (ASPB)
International Society for Plant Molecular Biology (ISPMB)
Sigma Xi
Ohio Academy of Science

Editorial Boards

Biology (Basel, Switzerland)
Pakistan Journal of Botany (Karachi, Pakistan)
BMC Plant Biology (New York, NY, USA)

Showalter Lab Students, Graduate Students and Advisees

Current Graduate Students, Undergraduate Students, and Post-Docs in the Showalter Lab

Xiao Liu (Ph.D. student)
Oyeyemi Ajayi (Ph.D. student)
Dasmeet Kaur (Ph.D. student)
Tristan Hoffman (undergraduate student)
Dylan Torok (undergraduate student)
David Masters (undergraduate student)
Dr. Melanie Schori (post-doc, completed lab work and now preparing 3 manuscript submissions)

Graduate Students Advised (19)

Graduate Student | Year | Degree | Dissertation/Thesis Title

Debarti Basu (2015) Ph.D. Identification and Characterization of Five Arabidopsis Hydroxyproline Galactosyltransferases and Their Functional Roles in Arabinogalactan-Protein Glycosylation, Growth, Development, and Cellular Signaling
Lu Tian (2015) M.S. Characterization of Genetic Mutants Encoding Four Hydroxyproline Galactosyltransferases (Hyp-GALTs) for Arabinogalactan-Proteins in Arabidopsis
Yan Liang (2012) Ph.D. Identification and Characterization of Galactosyltransferases and Fucosyltransferases Involved in Arabinogalactan-Protein Glycosylation
Brian D. Keppler (2010) M.S. IRX14 and IRX14-LIKE: Two Glycosyl Transferases involved in Glucuronoxylan Biosynthesis in Arabidopsis
Yizhu Zhang (2008) Ph.D. Functional Characterization of Lysine-rich Arabinogalactan-Proteins (AGPs) and an AG Peptide in Arabidopsis
Harjinder Sardar (2007) Ph.D. Molecular Interactions of Arabinogalactan-Proteins (AGPs) in Tobacco Bright Yellow-2 Cultured Cells and Functional Identification of Four Classical AGPs in Arabidopsis
Yang Jie (2007) Ph.D. Functional Identification of Three Lysine-Rich Arabinogalactan-Proteins (AGPs) in Arabidopsis
Sarah Bashore (2006) Ph.D. Characterization of a Spontaneous Phaseolus Vulgaris Mutant with the Ability to Selectively Restrict Nodulation
Wenxian Sun (2004) Ph.D. Structure, expression and function of the tomato LeAGP-1 arabinogalactan protein and its homologs in Arabidopsis
Ming Chen (2004) Ph.D. Programmed cell death induced by perturbing the function of arabinogalactan-protein by Yariv reagent
Li-Wen Wang (2002) Ph.D. Cloning and Characterization of Genes Related to Betaine, the Effect of Salt on Cell Death and Competition on Atriplex Prostrata
Ming-geng Gao (1999) Ph.D. Structure, localization and function of a modular, tomato arabinogalactan-protein
Ayyappan Nair (1997) Ph.D. A wound-inducible peroxidase and oxidative burst in carrots
Shu-Xia Li (1996) Ph.D. Isolation and characterization of genes and complementary DNAs encoding tomato arabinogalactan protein
Grattan Welch (1996) M.S. An analysis of glycine-rich protein and extensin genes in tomato (Lycopersicon esculentum) and potato (Solanum tumberosum L.)
Li-Wen Wang (1996) M.S. The effects of salinity on growth and ion content of Atriplex triangularis
Zhixiong Yuan (1994) M.S. Isolation and characterization of cDNA and genomic clones encoding potato tuber lectin and potato extensin
Tsai Tzong-Lin (1992) M.S. Isolation and characterization of potato tuber lectin and potato extensin cDNA clones
Jin Zhou (1991) Ph.D. Isolation and characterization of cDNA and genomic DNA clones encoding tomato hydroxyproline-rich glycoproteins

Post-Docs Advised (13)

Dr. Dominique Rumeau (France)
Dr. Adrian Butt (England)
Dr. John Crosby (USA)
Dr. Jinsong Sheng (USA)
Dr. Jim Thomas (USA)
Dr. Gabriella Scippa (Italy)
Dr. Hua Lu (USA)
Dr. Ines Chaves (Portugal)
Dr. Wenliang Xu (China)
Dr. Wei Tang (China)
Dr. Yizhu Zhang (China)
Dr. Yan Liang (China)
Dr. Melanie Schori (USA)

Funding

United States Department of Agriculture (USDA)
National Science Foundation (NSF)
Ohio Plant Biotechnology Consortium (OPBC)
Ohio University
Pakistan-US Science and Technology Cooperation Program

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